Patton wrote: If chain drive, would inspect chain for proper slack adjustment and assure it's properly lubed, and kink-free.
Good Fortune!
I stand corrected--it turns out that you were right. Your drive chain comment prompted me to re-check the rear pulley alignment, and I discovered that it was mis-aligned. After adjusting the pulley to proper alignment, the noise is gone. Thanks so much for pointing me in the right direction.